SIGLEC-5,SIGLEC-14 anticorps

N° du produit ABIN5540042

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, IF, IP, EIA et ICC. Il présente une réactivité envers Humain et Chimpanzé.

Aperçu rapide pour SIGLEC-5,SIGLEC-14 anticorps (ABIN5540042)

Antigène: SIGLEC-5,SIGLEC-14

Reactivité: Humain, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Clone: 1A5

Détail du produit

Specificité: This antibody recognises human SIGLEC5 / CD170. Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).

Purification: Affinity chromatography on Protein G

Immunogène: SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1/25-1/200, use 10 μL of this working dilution to label 10e6 cells in 100 μL. Immunofluorescence. Immunoprecipitation. ELISA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détail du antigène

Antigène: SIGLEC-5,SIGLEC-14

Autre désignation: siglec5,siglec14

Sujet: Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.